Abstract
This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.
MeSH terms
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Animals
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Cells, Cultured
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Chromosomes, Artificial, Bacterial / genetics
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Chromosomes, Artificial, Yeast / genetics
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DNA Primers
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DNA, Recombinant / administration & dosage*
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DNA, Recombinant / genetics*
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Embryo, Mammalian / cytology
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Gene Transfer Techniques*
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Genetic Markers
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Genetics, Medical
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Humans
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Membrane Fusion
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Mice
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Microinjections
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Plasmids / genetics
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Polymerase Chain Reaction
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Spheroplasts / genetics
Substances
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DNA Primers
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DNA, Recombinant
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Genetic Markers