Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system

Nat Struct Mol Biol. 2008 May;15(5):462-8. doi: 10.1038/nsmb.1426. Epub 2008 Apr 27.

Abstract

Gram-negative bacteria translocate various proteins including virulence factors across their outer membrane via type 2 secretion systems (T2SSs). T2SSs are thought to contain a pseudopilus, a subcomplex formed by one major and several minor pseudopilins. We report the crystal structure of the complex formed by three minor pseudopilins from enterotoxigenic Escherichia coli. The GspK-GspI-GspJ complex has quasihelical characteristics and an architecture consistent with a localization at the pseudopilus tip. The alpha-domain of GspK has a previously unobserved fold with an unexpected dinuclear metal binding site. The area surrounding its disulfide bridge is conserved and might interact with other T2SS components or with secreted proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Crystallography, X-Ray
  • Enterotoxigenic Escherichia coli / chemistry
  • Enterotoxigenic Escherichia coli / metabolism*
  • Escherichia coli Proteins / chemistry
  • Escherichia coli Proteins / metabolism*
  • Models, Molecular
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / metabolism
  • Protein Structure, Tertiary

Substances

  • Escherichia coli Proteins
  • GspI protein, E coli
  • GspJ protein, E coli
  • GspK protein, E coli
  • Multiprotein Complexes

Associated data

  • PDB/3CI0