Abstract
Bam32 (B lymphocyte adapter molecule of 32 kDa) is an adapter protein expressed in some hematopoietic cells including B and T lymphocytes. It was previously shown that Bam32-deficient mice have defects in various aspects of B cell activation including B cell receptor (BCR)-induced Erk activation, BCR-induced proliferation and T-independent antibody responses. In this study, we have examined the role of Bam32 in T cell activation using Bam32-deficient mice. By comparing CD4(+) T cells from lymph nodes of wild-type and Bam32-deficient mice, we found that Bam32 was required for optimal TCR-induced Erk activation, cytokine production, proliferation and actin-mediated spreading of CD4(+) T cells. These results indicate a novel pathway to Erk activation in T cells involving the adapter protein Bam32.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Actins / genetics
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Actins / immunology
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / immunology
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Adaptor Proteins, Signal Transducing / metabolism*
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Animals
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CD4-Positive T-Lymphocytes / metabolism*
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Cell Adhesion / immunology
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Cell Proliferation
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Cytokines / metabolism
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Enzyme Activation / genetics
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Extracellular Signal-Regulated MAP Kinases / immunology
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Extracellular Signal-Regulated MAP Kinases / metabolism*
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Humans
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Jurkat Cells
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Lipoproteins / genetics
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Lipoproteins / immunology
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Lipoproteins / metabolism*
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Lymphocyte Activation / genetics
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / metabolism
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Signal Transduction
Substances
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Actins
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Adaptor Proteins, Signal Transducing
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Cytokines
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Dapp1 protein, mouse
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Lipoproteins
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Receptors, Antigen, T-Cell
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Extracellular Signal-Regulated MAP Kinases