Abstract
Demethoxycurcumin and bisdemethoxycurcumin are the main active ingredients isolated from Curcumae Longae Radix. Recent studies demonstrated that both compounds exhibit antioxidative and anti-inflammatory effects as well as effects on cancer cell lines. In this study, we compared the activities of demethoxycurcumin and bisdemethoxycurcumin, and both compounds were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cycloxygenase-2 (COX-2) and nuclear factor-kappaB (NF-kappaB) activity in a RAW 264.7 macrophage cell line. The evaluation:results suggested that the anti-inflammatory properties of demethoxycurcumin and bisdemethoxycurcumin were attributed to the inhibition of iNOS and COX-2 expression, as initiated by the inhibition of NF-kappaB activity. Additionally, both of them significantly inhibited carrageenan-induced paw edema in mice. Taken together, all of the results showed that the suppressive effect of demethoxycurcumin was stronger than that of bisdemethoxycurcumin, indicating that the methoxy group had enhanced demethoxycurcumin's anti-inflammation effects.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Anti-Inflammatory Agents / therapeutic use
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Carrageenan
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Cell Line
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Curcumin / analogs & derivatives*
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Curcumin / pharmacology
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Curcumin / therapeutic use
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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Diarylheptanoids
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Edema / chemically induced
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Edema / metabolism
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Edema / prevention & control*
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Gene Expression Regulation, Enzymologic / drug effects
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I-kappa B Proteins / metabolism
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Inflammation Mediators / metabolism*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects*
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Macrophages / enzymology
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Macrophages / metabolism
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Mice
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NF-KappaB Inhibitor alpha
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / genetics
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Nitric Oxide Synthase Type II / metabolism
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Phosphorylation
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Transfection
Substances
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Anti-Inflammatory Agents
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Diarylheptanoids
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I-kappa B Proteins
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Inflammation Mediators
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Lipopolysaccharides
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NF-kappa B
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Nfkbia protein, mouse
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NF-KappaB Inhibitor alpha
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bisdemethoxycurcumin
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Nitric Oxide
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Carrageenan
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Ptgs2 protein, mouse
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Cyclooxygenase 2
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Curcumin
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demethoxycurcumin