Typing of SHV extended-spectrum beta-lactamases by pyrosequencing in Klebsiella pneumoniae strains with chromosomal SHV beta-lactamase

Marjo Haanperä; Sofia D Forssten; Pentti Huovinen; Jari Jalava

doi:10.1128/AAC.01259-07

Typing of SHV extended-spectrum beta-lactamases by pyrosequencing in Klebsiella pneumoniae strains with chromosomal SHV beta-lactamase

Antimicrob Agents Chemother. 2008 Jul;52(7):2632-5. doi: 10.1128/AAC.01259-07. Epub 2008 May 5.

Authors

Marjo Haanperä¹, Sofia D Forssten, Pentti Huovinen, Jari Jalava

Affiliation

¹ Laboratory of Human Microbial Ecology, Department of Bacterial and Inflammatory Diseases, National Public Health Institute, Kiinamyllynkatu 13, FIN-20520 Turku, Finland. [email protected]

Abstract

In Klebsiella pneumoniae, the cooccurrence of chromosomal and plasmid-mediated beta-lactamases can hinder their accurate molecular detection. We developed a fast and reliable method that allows the typing of isolates carrying more than one SHV gene. The method is based on pyrosequencing the DNA sequence corresponding to amino acid positions 35, 238, and 240.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Base Sequence
Chromosomes, Bacterial / genetics
DNA Primers / genetics
DNA, Bacterial / genetics
Genes, Bacterial
Humans
Klebsiella pneumoniae / classification
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Point Mutation
Sequence Analysis, DNA
beta-Lactamases / classification
beta-Lactamases / genetics*

Substances

DNA Primers
DNA, Bacterial
beta-Lactamases