The purpose of the study was to translate and adapt in the Italian language a questionnaire that could effectively assess perceived motivational climate in physical education. An Italian version of the Teacher-initiated Motivational Climate in Physical Education Questionnaire proposed by Papaioannou (1998) was administered to 1,681 young students, 840 girls and 841 boys, ages 11 to 14 years. Data were subjected to confirmatory factor analysis to assess the goodness of fit of the expected two-factor solution constituted by a Mastery Climate scale (six items) and a Performance Climate scale (six items). A good fit was indicated between the observed data and theoretical model and supported the two-dimensional structure of the test. In the total sample, the Mastery factor accounted for 33.6% of the variance and the Performance factor accounted for 15.4%. Cronbach alpha of the Mastery and Performance scales were .79 and .70, respectively.