Abstract
Methylphosphonate (PC) backbone oligodeoxynucleotides complementary to the 5'-terminal nucleotides of U1 and U2 small nuclear (sn) RNAs do not elicit RNase H action under conditions in which natural (phosphodiester) oligodeoxynucleotides yield extensive RNase H cleavage. We show here that antisense PC oligonucleotides can mask sites in U1 and U2 snRNPs that are required for spliceosome formation. We further report that biotinylated derivatives of antisense PC oligos can be used for affinity selection of U1 and U2 snRNPs.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Biotin
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Cell Nucleus / metabolism
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Endoribonucleases / metabolism
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HeLa Cells / metabolism
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Humans
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Molecular Sequence Data
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Oligonucleotides, Antisense / chemical synthesis
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Oligonucleotides, Antisense / pharmacology*
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Organophosphorus Compounds*
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RNA Splicing / drug effects*
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RNA, Neoplasm / drug effects
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RNA, Neoplasm / metabolism
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Ribonuclease H
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Ribonucleoproteins / metabolism*
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Ribonucleoproteins, Small Nuclear
Substances
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Oligonucleotides, Antisense
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Organophosphorus Compounds
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RNA, Neoplasm
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Ribonucleoproteins
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Ribonucleoproteins, Small Nuclear
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methylphosphonic acid
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Biotin
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Endoribonucleases
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Ribonuclease H