Abstract
Cytogenetic and molecular genetic studies of an intersex horse have been carried out. The investigated animal had overall male body conformation; however, its external genitalia consisted of incompletely developed vulva and penis. The X and Y chromosome painting probes detected three cell lines in the examined horse: 63,X, 64,XX and 65,XX with a fragment of a Y chromosome (del Y). The DNA analysis with the PCR and PCR/RFLP methods showed absence of SRY,AMELY and ZFY genes as well as of six Y microsatellite markers (YM2, YP9, YJ10, YE1, YH12, and YA16). These results suggest that the Y chromosome fragment detected in the investigated animal was the result of a deletion of a euchromatic fragment comprising the above-mentioned markers.
Copyright 2008 S. Karger AG, Basel.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Chromosome Painting / veterinary
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DNA Primers / genetics
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Disorders of Sex Development / genetics
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Disorders of Sex Development / veterinary*
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Female
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Forkhead Transcription Factors / genetics
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Genes, sry
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High Mobility Group Proteins / genetics
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Horse Diseases / genetics*
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Horses / genetics*
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In Situ Hybridization, Fluorescence / veterinary
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Karyotyping / veterinary
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Male
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Mosaicism / veterinary
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Polymerase Chain Reaction / veterinary
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SOX9 Transcription Factor
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Sex Chromosome Aberrations / veterinary*
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Sex Differentiation / genetics
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Transcription Factors / genetics
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X Chromosome / genetics
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Y Chromosome / genetics
Substances
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DNA Primers
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Forkhead Transcription Factors
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High Mobility Group Proteins
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SOX9 Transcription Factor
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Transcription Factors