Schistosoma mansoni: characterization and identification of calcium-binding proteins associated with the apical plasma membrane and envelope

A A Siddiqui; R B Podesta; M W Clarke

doi:10.1016/0014-4894(91)90121-c

Schistosoma mansoni: characterization and identification of calcium-binding proteins associated with the apical plasma membrane and envelope

Exp Parasitol. 1991 Jan;72(1):63-8. doi: 10.1016/0014-4894(91)90121-c.

Authors

A A Siddiqui¹, R B Podesta, M W Clarke

Affiliation

¹ Department of Zoology, University of Western Ontario, London, Canada.

PMID: 1847106
DOI: 10.1016/0014-4894(91)90121-c

Abstract

Calcium-binding proteins (CaBPs) of Schistosoma mansoni were purified by hydrophobic affinity chromatography. Metabolically labeled CaBPs were characterized using SDS-polyacrylamide gel electrophoresis followed by fluorography. A number of CaBPs were detected in total tissue extracts, apical plasma membrane, and soluble fractions of the apical bilayer complex, ranging from 15 to 205 kDa in their molecular masses. No CaBPs were discerned in the envelope of the apical bilayer complex. Two CaBPs were positively identified as calmodulin and gelsolin via immunoblot analyses. The possible role of CaBPs in surface signal transduction mechanisms has also been briefly discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
Calcium-Binding Proteins / analysis*
Calcium-Binding Proteins / chemistry
Calcium-Binding Proteins / isolation & purification
Calmodulin / analysis
Calmodulin / chemistry
Calmodulin / isolation & purification
Cell Membrane / chemistry
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Gelsolin
Microfilament Proteins / analysis
Microfilament Proteins / chemistry
Microfilament Proteins / isolation & purification
Molecular Weight
Schistosoma mansoni / analysis*
Schistosoma mansoni / ultrastructure
Signal Transduction

Substances

Calcium-Binding Proteins
Calmodulin
Gelsolin
Microfilament Proteins