Induction of a cyclic AMP-responsive gene in living cells requires the nuclear factor CREB

Mol Cell Biol. 1991 Mar;11(3):1759-64. doi: 10.1128/mcb.11.3.1759-1764.1991.

Abstract

We constructed cell lines containing various enhancer elements cloned upstream from a marker gene. By microinjection of specific antibodies directed against transcriptional activator proteins into these cell lines, we have developed a functional assay for factors which regulate the activity of target promoters. Here we show that microinjection of a highly specific antibody to the cyclic AMP enhancer element-binding (CREB) protein diminishes gene expression in response to cyclic AMP in living fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cloning, Molecular
  • Cyclic AMP / physiology*
  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins / physiology*
  • Enhancer Elements, Genetic
  • Gene Expression Regulation*
  • Immunologic Techniques
  • Microinjections
  • Rats

Substances

  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins
  • Cyclic AMP