Identification of a third alternatively spliced cDNA encoding the catalytic subunit of protein phosphatase 2B beta

A E McPartlin; H M Barker; P T Cohen

doi:10.1016/0167-4781(91)90069-x

Identification of a third alternatively spliced cDNA encoding the catalytic subunit of protein phosphatase 2B beta

Biochim Biophys Acta. 1991 Feb 16;1088(2):308-10. doi: 10.1016/0167-4781(91)90069-x.

Authors

A E McPartlin¹, H M Barker, P T Cohen

Affiliation

¹ Department of Biochemistry, The University, Dundee, U.K.

PMID: 1848109
DOI: 10.1016/0167-4781(91)90069-x

Abstract

Complementary DNA coding for a catalytic subunit of protein phosphatase 2B was isolated from a human teratocarcinoma library. It encodes a third isoform of protein phosphatase 2B beta, and differs from the cDNA for the second isoform by a deletion of 30 base pairs in the coding region. The deletion results in the loss of ten amino acids between the putative calmodulin site and a postulated autoinhibitory domain. An identical deletion occurs in one of the two alternatively spliced isoforms of PP2B alpha.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Brain / enzymology
Calcineurin
Calmodulin-Binding Proteins / genetics*
Cloning, Molecular
DNA, Neoplasm / genetics*
Gene Library
Humans
Macromolecular Substances
Molecular Sequence Data
Oligonucleotide Probes
Phosphoprotein Phosphatases / genetics*
RNA Splicing*
Sequence Homology, Nucleic Acid
Teratoma / enzymology

Substances

Calmodulin-Binding Proteins
DNA, Neoplasm
Macromolecular Substances
Oligonucleotide Probes
Calcineurin
Phosphoprotein Phosphatases