Abstract
In Saccharomyces cerevisiae, Rad52 plays major roles in several types of homologous recombination. Here, we found that rad52-K200R mutation greatly reduced sumoylation of Rad52. The rad52-K200R mutant exhibited defects in various types of recombination, such as intrachromosomal recombination and mating-type switching. The K200 residue of Rad52 is part of the nuclear localization signal (NLS), which is important for transport into the nucleus. Indeed, the addition of a SV40 NLS to Rad52-K200R suppressed the sumoylation defect of Rad52-K200R. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Amino Acid Substitution
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Arginine / genetics
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Arginine / metabolism
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Cell Nucleus / chemistry
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Cell Nucleus / metabolism*
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Chromatids / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Lysine / genetics
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Lysine / metabolism
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Mutation
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Nuclear Localization Signals / genetics
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Protein Processing, Post-Translational*
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Rad52 DNA Repair and Recombination Protein / analysis
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Rad52 DNA Repair and Recombination Protein / genetics
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Rad52 DNA Repair and Recombination Protein / metabolism*
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Recombination, Genetic
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / analysis
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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Nuclear Localization Signals
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RAD52 protein, S cerevisiae
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Rad52 DNA Repair and Recombination Protein
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Saccharomyces cerevisiae Proteins
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Arginine
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SCEI protein, S cerevisiae
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Deoxyribonucleases, Type II Site-Specific
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Lysine