[Usefulness of the determination of IgG and IgM antibodies by ELISA and immunocapture in a clinical series of human brucellosis]

Rev Chilena Infectol. 2008 Apr;25(2):116-21. Epub 2008 Apr 30.
[Article in Spanish]

Abstract

Introduction: The diagnostic difficulties of brucellosis makes the evaluation of new diagnostic tests necessary.

Objectives: Evaluation of different commercial tests in the serological diagnosis of brucellosis by ELISA and immunocapture antibodies in a clinical series of patients with brucellosis of the Health Network of the Catholic University of Chile.

Methods: All the serums received in the Laboratory of Microbiology for suspicion of brucellosis during five years were studied. Two groups were obtained, one that fulfilled diagnostic criteria for brucellosis [clinical evidence, and/or positive blood culture and/or seroagglutination test (SAT) in titers > 1/160] and the control group. Each serum sample was analyzed using irnmunocapture-agglutination test (Brucellacapt), ELISA IgM and IgG.

Results: Of 10 patients with brucellosis, the serologic results were: 8/10 positives for ELISA IgG, 7/10 for Brucellacapt and SAT, and 5/10 for ELISA IgM.

Discussion: ELISA IgG alone was superior than SAT. The combination ELISA IgG/ Brucellacapt reaches the best detection performance (9/10) and can be an alternative to SAT.

Publication types

  • Evaluation Study

MeSH terms

  • Acute Disease
  • Adult
  • Aged
  • Agglutination Tests / methods*
  • Brucella / immunology*
  • Brucellosis / diagnosis*
  • Brucellosis / immunology
  • Case-Control Studies
  • Chronic Disease
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Male
  • Middle Aged
  • Reproducibility of Results
  • Retrospective Studies

Substances

  • Immunoglobulin G
  • Immunoglobulin M