Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

J Immunol Methods. 2008 Jul 20;336(1):71-7. doi: 10.1016/j.jim.2008.04.003. Epub 2008 Apr 28.

Abstract

Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, few reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cell-mediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Bronchoalveolar Lavage Fluid / virology
  • Cross Reactions
  • Disease Models, Animal
  • Ferrets / immunology
  • Ferrets / virology*
  • Flow Cytometry
  • Immunity, Cellular / immunology
  • Influenza A virus / immunology*
  • Orthomyxoviridae Infections / diagnosis*
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / virology
  • Specific Pathogen-Free Organisms
  • Spleen / immunology
  • Spleen / virology

Substances

  • Antibodies, Monoclonal