Characterization of microglial attachment and cytokine release on biomaterials of differing surface chemistry

Biomaterials. 2008 Aug;29(23):3289-97. doi: 10.1016/j.biomaterials.2008.03.045. Epub 2008 May 16.

Abstract

The clinical usefulness of central nervous system recording electrodes is currently limited by inconsistent long-term performance that is believed to be governed by the brain tissue response to the implant. In this study, we observed persistent macrophage biomarker expression at the biotic-abiotic interface surrounding implanted electrodes over a 12-week indwelling period. Using the cell type-specific marker CD11b to examine the cells attached to electrodes retrieved over the indwelling period, we found that most of the cells were activated microglia, the resident macrophage of brain tissue, indicating that the implanted electrodes behave as a persistent inflammatory stimulus. To determine the potential usefulness of different materials as coatings for implanted electrodes, we examined brain-derived microglial cell attachment and cytokine release on a number of medically relevant materials. Our results suggest that activated microglia attach to many of the materials used as external coatings for electrode manufacture, and likely serve as a source of pro-inflammatory and neurotoxic cytokines that may be responsible for reducing the biocompatibility of such implants. Our results also indicate that low protein-binding coatings may be useful in reducing microglial attachment upon implantation in brain tissue and may provide a means of improving electrode biocompatibility.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Brain / cytology
  • Cell Adhesion
  • Cells, Cultured
  • Cytokines / biosynthesis*
  • Electrodes, Implanted
  • Materials Testing
  • Microglia / cytology*
  • Microglia / physiology*
  • Protein Binding
  • Rats
  • Surface Properties

Substances

  • Biocompatible Materials
  • Cytokines