Abstract
In an evolutionarily conserved gene organization (syntenic region), the sigH gene shares exceptionally low homology among staphylococcal species. We analyzed the "positionally cloned" sigH sequences of 39 staphylococcal species. The topology of the SigH phylogenetic tree was consistent with that of 16S rRNA. Certain clinical isolates were successfully differentiated at the species level with the sigH sequence data set. We propose that the sigH gene is a promising molecular target in genotypic identification because it is highly discriminative in differentiating closely related staphylococcal species.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Typing Techniques / methods*
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DNA, Bacterial / genetics
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Molecular Sequence Data
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Phylogeny
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Polymorphism, Genetic
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Sigma Factor / genetics*
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Staphylococcus / classification*
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Staphylococcus / genetics*
Substances
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Bacterial Proteins
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DNA, Bacterial
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RNA, Ribosomal, 16S
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SigH protein, bacteria
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Sigma Factor
Associated data
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GENBANK/EU296744
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GENBANK/EU296745
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GENBANK/EU296746
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GENBANK/EU296747
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GENBANK/EU296748
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GENBANK/EU296749
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GENBANK/EU296750
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GENBANK/EU296751
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GENBANK/EU296752
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GENBANK/EU296753
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GENBANK/EU296754
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GENBANK/EU296755
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GENBANK/EU296756
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GENBANK/EU296757
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GENBANK/EU296758
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GENBANK/EU296759
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GENBANK/EU296760
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GENBANK/EU296761
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GENBANK/EU296762
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GENBANK/EU296763
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GENBANK/EU296764
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GENBANK/EU296765
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GENBANK/EU296766
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GENBANK/EU296767
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GENBANK/EU296768
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GENBANK/EU296769
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GENBANK/EU296770
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GENBANK/EU296771