Glutathione transferase pi its minimal promoter and downstream cis-acting element

Biochem Biophys Res Commun. 1991 Apr 15;176(1):233-40. doi: 10.1016/0006-291x(91)90914-s.

Abstract

Fragments of the human glutathione S-transferase pi gene and 15 kb of its 5' flanking region have been fused to the chloramphenicol acetyl transferase (CAT) reporter gene. Transfection into a number of human cell lines (Hela, HepG2, MCF7 and EJ) has demonstrated that the AP1 binding site, located between nucleotides -58 and -65 (Cowell et al. 1988. Biochem. J. 255, 79-83), is essential for basal level promoter activity. We have also identified a positive cis-acting DNA element between nucleotides +8 and +72 which seems to be part of the promoter. No other regulatory activity was identified within the 17 kb analyzed.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Breast Neoplasms
  • Carcinoma, Hepatocellular
  • Cell Line
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • DNA-Binding Proteins / metabolism
  • Exons
  • Female
  • Glutathione Transferase / genetics*
  • Glutathione Transferase / metabolism
  • HeLa Cells / enzymology
  • Humans
  • Introns
  • Isoenzymes / genetics*
  • Isoenzymes / metabolism
  • Liver Neoplasms
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Plasmids
  • Promoter Regions, Genetic*
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins c-jun
  • Rats
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / metabolism
  • Transfection
  • Urinary Bladder Neoplasms

Substances

  • DNA-Binding Proteins
  • Isoenzymes
  • Oligonucleotide Probes
  • Proto-Oncogene Proteins c-jun
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Chloramphenicol O-Acetyltransferase
  • Glutathione Transferase
  • Protein-Tyrosine Kinases