VIM-15 and VIM-16, two new VIM-2-like metallo-beta-lactamases in Pseudomonas aeruginosa isolates from Bulgaria and Germany

Ines Schneider; Emma Keuleyan; Rudolf Rasshofer; Rumyana Markovska; Anne Marie Queenan; Adolf Bauernfeind

doi:10.1128/AAC.00175-08

VIM-15 and VIM-16, two new VIM-2-like metallo-beta-lactamases in Pseudomonas aeruginosa isolates from Bulgaria and Germany

Antimicrob Agents Chemother. 2008 Aug;52(8):2977-9. doi: 10.1128/AAC.00175-08. Epub 2008 Jun 2.

Authors

Ines Schneider¹, Emma Keuleyan, Rudolf Rasshofer, Rumyana Markovska, Anne Marie Queenan, Adolf Bauernfeind

Affiliation

¹ MICOER-Institute, Hesseloherstrasse 4, 80802 Munich, Germany.

Abstract

Two Pseudomonas aeruginosa urine isolates from Bulgaria and Germany produced two new VIM-2 variants. VIM-15 had one amino acid substitution (Tyr218Phe) which caused a significant increase in hydrolytic efficiency. The substitution Ser54Leu, characterizing VIM-16, showed no influence on enzyme activity. Both genes were part of class I integrons located in the chromosome.

MeSH terms

Amino Acid Substitution
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Bulgaria
Catalysis
Germany
Hydrolysis
Isoenzymes / genetics
Isoenzymes / metabolism
Kinetics
Microbial Sensitivity Tests
Molecular Sequence Data
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / isolation & purification*
Sequence Analysis, DNA
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Bacterial Proteins
Isoenzymes
beta-Lactamases

Associated data

GENBANK/EU419745
GENBANK/EU419746