Fluorimetric detection of aldehyde dehydrogenase activity in human tissues in diagnostic of cancers of oral cavity

Acta Pol Pharm. 2008 Jan-Feb;65(1):81-4.

Abstract

Aldehyde dehydrogenase (ALDH) is known to be susceptible to oxidation, and its assays require stabilization of the enzyme by thiols. Application of the fluorimetric method to assay the ALDH activity in human saliva demonstrated significant differences between procedures utilizing glutathione (GSH) and dithiothreitol (DTT) as stabilizing agents. It has been recently shown that average aldehyde dehydrogenase (ALDH3A1) activity in cancerous (oral cavity cancer) patients' tissues was higher than that found in the control group, what may indicate induction of tumor-specific ALDH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aldehyde Dehydrogenase / analysis*
  • Aldehyde Dehydrogenase / metabolism
  • Dithiothreitol / metabolism
  • Female
  • Fluorometry / methods*
  • Glutathione / metabolism
  • Humans
  • Male
  • Middle Aged
  • Mouth Neoplasms / diagnosis*
  • Oxidation-Reduction

Substances

  • ALDH3A1 protein, human
  • Aldehyde Dehydrogenase
  • Glutathione
  • Dithiothreitol