Licochalcone A inhibits the formation and bone resorptive activity of osteoclasts

Soon Nam Kim; Myung Hee Kim; Yong Ki Min; Seong Hwan Kim

doi:10.1016/j.cellbi.2008.04.017

Licochalcone A inhibits the formation and bone resorptive activity of osteoclasts

Cell Biol Int. 2008 Sep;32(9):1064-72. doi: 10.1016/j.cellbi.2008.04.017. Epub 2008 May 2.

Authors

Soon Nam Kim¹, Myung Hee Kim, Yong Ki Min, Seong Hwan Kim

Affiliation

¹ Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Department of Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.

PMID: 18539489
DOI: 10.1016/j.cellbi.2008.04.017

Abstract

Licochalcone A on the formation and bone resorptive activity of osteoclasts up to 5muM significantly inhibited the receptor activator of nuclear factor kappaB (NF-kappaB) ligand (RANKL)-induced activity of tartrate-resistant acid phosphatase (TRAP) activity and formation of osteoclasts without any effect on cell viability. Interestingly, licochalcone A was shown to inhibit the RANKL-induced activation of extracellular signal-regulated kinase, translocation of NF-kappaB into nucleus and mRNA expression of Fra-2. Licochalcone A also inhibited the bone resorptive activity of mature osteoclasts and the expression of bone resorption-related genes. Inhibitory effects of licochalcone A on the formation and bone resorptive activity of mouse bone marrow macrophage-derived osteoclasts were also observed. In conclusion, licochalcone A has the potential to inhibit the formation of osteoclasts as well as the bone resorptive activity of mature osteoclasts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Resorption / pathology*
Cell Survival / drug effects
Cells, Cultured
Chalcones / chemistry
Chalcones / pharmacology*
Enzyme Activation / drug effects
Gene Expression Regulation / drug effects
I-kappa B Proteins / metabolism
Macrophage Colony-Stimulating Factor / pharmacology
Mice
Mice, Inbred BALB C
Mitogen-Activated Protein Kinases / metabolism
NF-KappaB Inhibitor alpha
NFATC Transcription Factors / genetics
NFATC Transcription Factors / metabolism
Osteoclasts / drug effects*
Osteoclasts / enzymology
Osteoclasts / pathology*
RANK Ligand / pharmacology
RNA, Messenger / genetics
RNA, Messenger / metabolism
Transcription Factor AP-1 / genetics
Transcription Factor AP-1 / metabolism
Transcription Factor RelA / metabolism

Substances

Chalcones
I-kappa B Proteins
NFATC Transcription Factors
Nfkbia protein, mouse
RANK Ligand
RNA, Messenger
Transcription Factor AP-1
Transcription Factor RelA
NF-KappaB Inhibitor alpha
Macrophage Colony-Stimulating Factor
Mitogen-Activated Protein Kinases
licochalcone A