Toxicological screening of drugs by microbore high-performance liquid chromatography with photodiode-array detection and ultraviolet spectral library searches

Clin Chem. 1991 Jul;37(7):1210-5.

Abstract

We use ultraviolet data, acquired with a photodiode-array detector coupled to a reversed-phase liquid-chromatographic system, to identify unknown drugs in plasma samples of acutely poisoned patients. Both retention time and spectra of the peaks obtained with a microbore Hypersil ODS column under gradient elution are compared with a library of approximately 350 compounds. We present our three-year experience with this system, which identifies drugs in less than 1 h, with a high degree of confidence.

MeSH terms

  • Chromatography, High Pressure Liquid*
  • Data Interpretation, Statistical
  • Humans
  • Pharmaceutical Preparations / analysis*
  • Poisoning / diagnosis*
  • Spectrophotometry, Ultraviolet*

Substances

  • Pharmaceutical Preparations