Abstract
Peptide release on the ribosome is catalyzed by protein release factors (RFs) on recognition of stop codons positioned in the A site of the small ribosomal subunit. Here we show that the 2' OH of the peptidyl-tRNA substrate plays an essential role in catalysis of the peptide release reaction. These observations parallel earlier studies of the mechanism of the peptidyl transfer reaction and argue that related mechanisms are at the heart of catalysis for these reactions.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Peptide Chain Termination, Translational*
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Peptide Termination Factors / metabolism
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Peptides / metabolism*
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Protein Biosynthesis
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RNA, Transfer, Amino Acyl / chemistry
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RNA, Transfer, Amino Acyl / metabolism*
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Ribosomes / metabolism*
Substances
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Peptide Termination Factors
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Peptides
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RNA, Transfer, Amino Acyl
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tRNA, peptidyl-