[Protective effect of hypotensive fluid resuscitation against lung ischemia-reperfusion injury in pregnant rabbits with uncontrolled hemorrhagic shock]

Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jun;28(6):1042-4.
[Article in Chinese]

Abstract

Objective: To evaluate the effects of different fluid resuscitation strategies on superoxide dismutase (SOD), malondialdehyde (MDA) and myeloperoxidase (MPO) activities in the lung tissue in pregnant rabbits with uncontrolled hemorrhagic shock.

Methods: Thirty pregnant New Zealand rabbits were randomized into 5 equal groups, namely the sham shock.(SS) group, shock group without interventions (SH group), and hemorrhagic shock groups with conventional normal saline (NS) resuscitation, NS hypotensive resuscitation, and hypertonic hyperosmotic hypotensive resuscitation (NS, NH, HHH groups, respectively) 30 min after the shock. At the end of the experiment, the rabbits were sacrificed, and the lungs were taken for detection of MDA, MPO and SOD levels.

Results: Ischemia-reperfusion injury of the lungs in uncontrolled hemorrhagic shock resulted in decreased SOD and increased MDA and MPO contents. The MDA and MPO contents in HHH group were significantly lower than those in NH group, and both the groups, MDA and MPO contents were significantly lower than those of NS group (P<0.05). SOD activity was significantly higher in HHH group than in NH group (P<0.05).

Conclusion: In pregnant rabbits with uncontrolled hemorrhagic shock, hypotensive resuscitation more effectively ameliorates ischemia-reperfusion injuries in the lungs than aggressive fluid resuscitation, and hyperosmotic crystalloid and hyperonoctic colloid resuscitation provide significant protective effects against such injuries.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Fluid Therapy / methods
  • Hypertonic Solutions / therapeutic use
  • Hypotonic Solutions / therapeutic use
  • Lung / blood supply*
  • Lung / enzymology
  • Peroxidase / metabolism
  • Pregnancy
  • Pregnancy Complications / therapy*
  • Rabbits
  • Random Allocation
  • Reperfusion Injury / prevention & control*
  • Resuscitation / methods
  • Shock, Hemorrhagic / complications
  • Shock, Hemorrhagic / therapy*
  • Superoxide Dismutase / metabolism

Substances

  • Hypertonic Solutions
  • Hypotonic Solutions
  • Peroxidase
  • Superoxide Dismutase