Abstract
Yeast Mot1p, a member of the Snf2 ATPase family of proteins, is a transcriptional regulator that has the unusual ability to both repress and activate mRNA gene transcription. To identify interactions with other proteins that may assist Mot1p in its regulatory processes, Mot1p was purified from replicate yeast cell extracts, and Mot1p-associated proteins were identified by coupled multidimensional liquid chromatography and tandem mass spectrometry. Using this approach we generated a catalog of Mot1p-interacting proteins. Mot1p interacts with a range of transcriptional co-regulators as well as proteins involved in chromatin remodeling. We propose that interaction with such a wide range of proteins may be one mechanism through which Mot1p subserves its roles as a transcriptional activator and repressor.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenosine Triphosphatases
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Base Sequence
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Chromatin Assembly and Disassembly
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DNA Helicases / chemistry
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DNA Helicases / genetics
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DNA Helicases / isolation & purification
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DNA Helicases / metabolism*
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DNA, Fungal / genetics
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DNA, Fungal / metabolism
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Models, Molecular
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Molecular Weight
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Multiprotein Complexes / chemistry
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Multiprotein Complexes / genetics
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Multiprotein Complexes / isolation & purification
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Multiprotein Complexes / metabolism
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Plasmids / genetics
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Protein Interaction Domains and Motifs
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Protein Interaction Mapping
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Proteomics
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / isolation & purification
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Saccharomyces cerevisiae Proteins / metabolism*
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TATA-Binding Protein Associated Factors / chemistry
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TATA-Binding Protein Associated Factors / genetics
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TATA-Binding Protein Associated Factors / isolation & purification
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TATA-Binding Protein Associated Factors / metabolism*
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Tandem Mass Spectrometry
Substances
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DNA, Fungal
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Multiprotein Complexes
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Saccharomyces cerevisiae Proteins
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TATA-Binding Protein Associated Factors
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Adenosine Triphosphatases
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MOT1 protein, S cerevisiae
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DNA Helicases