Abstract
VEGF-induced Ca2+ signalling was investigated in CD133+/VEGFR-2+ progenitor cells isolated from human adipose stroma. Colonies derived from CD133+ immunoselected cells displayed inhomogenous Ca2+ signals, with variable magnitude of VEGF-induced Ca2+ entry, which positively correlated with expression of the Ca2+ channel protein TRPC3. High levels of VEGF-induced Ca2+ entry and TRPC3 expression were preferentially detected in rim areas of expanding colonies. Dominant negative suppression of TRPC3 inhibited VEGF-induced Ca2+ entry into CD133+ cells. Our results identify TRPC3 as a key Ca2+ entry channel in a subset of CD133+ stem cells. We suggest TRPC3 as an essential determinant of cell fate in CD133+ progenitor-derived colonies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AC133 Antigen
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Adipose Tissue / cytology*
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Adipose Tissue / drug effects
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Adipose Tissue / metabolism
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Adult
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Aged
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Aged, 80 and over
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Antigens, CD / biosynthesis*
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Calcium Channels / biosynthesis*
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Calcium Channels / genetics
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Cell Differentiation
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Cell Line
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Cells, Cultured
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Female
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Glycoproteins / biosynthesis*
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Humans
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Male
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Middle Aged
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Neovascularization, Physiologic
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Peptides
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Stem Cells / cytology*
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Stem Cells / drug effects
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Stem Cells / metabolism
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TRPC Cation Channels / biosynthesis*
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TRPC Cation Channels / genetics
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Vascular Endothelial Growth Factor A / metabolism*
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Vascular Endothelial Growth Factor A / pharmacology
Substances
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AC133 Antigen
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Antigens, CD
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Calcium Channels
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Glycoproteins
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PROM1 protein, human
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Peptides
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TRPC Cation Channels
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TRPC3 cation channel
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Vascular Endothelial Growth Factor A