This is a comparative study on three groups. With the help of a coupling reagent Sulfo-LC-SPDP, the biological valve scaffolds were surface modified with one of arginine -glycine -aspartic acid (RGD) containing peptides by covalent bond(the treated group). After rat aortic myofibroblast seeding, MTT test showed that more cells of the treated group attached on the valve scaffolds coupled with RGD peptides when compared with the cells of the coated group and untreated group. Moreover, correlatioins of attachment with attaching time and peptide concentrations were observed. Light and electron microscopy and cell count also confirmed the findings. Therefore, immobilizing the RGD peptides on the decellularized valve scaffolds is effective for improving cell attachment, which is helpful to constructing tissue engineering heart valve.