Objective: For the protection of Changium smyrnioides Wolff germ-plasm resources.
Methods: Rapid propagation was conducted by tissue culture.
Results: The best explants were leaves. The optimum culture medium for the induction of callus was MS + 2,4-D 1.0 mg/L + Kt 1.0 mg/L; that of bud was MS + 6-BA 3.0 mg/L + NAA 0.2 mg/L; and that of root was MS + IBA 0.4 mg/L or MS + NAA 0.4 mg/L.
Conclusion: Rapid propagation of Changium smyrnioides is established with tissue culture, which provides an effective way for the sustainable utilization.