Abstract
Chronic administration of D-galactose (D-gal) is a useful method for establishing a model of natural aging. However, whether animal age or jet lag influences the D-gal-induced aging process has not been investigated. To clarify these issues, ICR male mice were given a daily injection of D-gal (500 mg/kg bw) for 7 wks. D-gal-treatment caused a significant decrease only in the transcript level of Cat gene in the 6-wk-old mice, whereas it significantly reduced the expression of Cat and Sod1 genes and of Cat, Gpx1, Sod1 and Sod2 genes in the 12- and 22-wk-old group, respectively, suggesting that D-gal induced aging process was greatly dependent on the age of mice. Mice receiving both D-gal and jet lag exhibited larger decreases in the expression levels of above four genes in liver as compared with that of either group singly, indicating a synergistic effect of jet lag and D-gal on the aging process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Age Factors
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Aging / drug effects*
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Aging / genetics
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Aging / metabolism
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Animals
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Catalase / genetics
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Galactose / administration & dosage
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Galactose / pharmacology*
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Gene Expression Regulation, Enzymologic / drug effects
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Glucuronidase / genetics
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Glutathione Peroxidase / genetics
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Glutathione Peroxidase GPX1
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Injections, Subcutaneous
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Jet Lag Syndrome / enzymology
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Jet Lag Syndrome / genetics
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Jet Lag Syndrome / physiopathology*
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Klotho Proteins
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Liver / drug effects
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Liver / enzymology
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Male
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Mice
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Mice, Inbred ICR
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Models, Animal
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Oxidative Stress / drug effects
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Oxidative Stress / genetics
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Photoperiod*
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Superoxide Dismutase / genetics
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Superoxide Dismutase-1
Substances
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RNA, Messenger
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Catalase
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Glutathione Peroxidase
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Sod1 protein, mouse
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Superoxide Dismutase
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Superoxide Dismutase-1
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superoxide dismutase 2
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Glucuronidase
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Klotho Proteins
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Galactose
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Glutathione Peroxidase GPX1
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Gpx1 protein, mouse