Use of tissue ink to maintain identification of individual cores on needle biopsies of the prostate

J Clin Pathol. 2008 Sep;61(9):1055-7. doi: 10.1136/jcp.2008.058545. Epub 2008 Jul 19.

Abstract

Background: There is an increasing necessity to extract the maximum amount of information, beyond even a cancer diagnosis, from prostate biopsies. Thus, maintaining site-specific information regarding individual biopsy cores might be critical.

Aim: To evaluate the applicability of employing tissue ink to maintain the identity of individual prostatic biopsy cores.

Method: In this ongoing study, 12 core prostate biopsy specimens are sent to the laboratory in individual pots labelled according to anatomical site. The specimens are placed in two separate multi-compartment cassettes. They are inked with different colours to identify the site of origin from each lobe. The cassettes are then processed with a single paraffin block for each side; the six cores from each side can be mounted on a single slide.

Results: The different colours used adhere well to the biopsy cores, thus maintaining the identity of each core. Six cores from each side are embedded in a single paraffin block and examined on a single slide, making it cost-effective, while maintaining high quality, accurate histopathological information.

Conclusion: Differential inking of prostate biopsy cores is an easily applicable method that is cost-effective and provides tumour location information. Prostate biopsy data archived to maintain individual core information might be used to determine applicability of such information to predict extra-capsular extension by correlating with imaging and radical prostatectomy findings, and for treatment planning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biopsy, Needle / methods*
  • Coloring Agents
  • Humans
  • Immunohistochemistry
  • Ink
  • Male
  • Middle Aged
  • Prostate / pathology*
  • Prostatic Neoplasms / pathology*
  • Staining and Labeling / methods*

Substances

  • Coloring Agents