Rosiglitazone-enriched diet did not protect liver ischemia-reperfusion injury in a rat model

Acta Cir Bras. 2008 Jul-Aug;23(4):378-83. doi: 10.1590/s0102-86502008000400013.

Abstract

Purpose: To determine whether rosiglitazone-enriched diet offer protection in a classical model of liver ischemia-reperfusion injury in rats.

Methods: Two days before the experiment, rats were divided into 2 groups: Control Group (n=13) rats fed with standard diet; Rosi Group (n=13): rats fed with a powdered standard diet supplemented with rosiglitazone. The animals were submitted to liver ischemia-reperfusion by clamping the pedicle of median and left anterolateral lobes. After 1 hour of partial hepatic ischemia, the clamp was removed for reperfusion. After 2 or 24 hours (Control and Rosi Groups), blood was collected for enzymes and cytokines analysis. Ischemic and non-ischemic liver were collected for malondialdehyde analysis and histological assessment. Lungs were removed for tissue myeloperoxidase quantification.

Results: There were no statistical differences between groups for all analysed parameters.

Conclusion: In this model, rosiglitazone-enriched diet did not protect liver against ischemia-reperfusion injury.

MeSH terms

  • Animals
  • Aspartate Aminotransferases / blood
  • Cytokines / blood
  • Dietary Supplements*
  • Disease Models, Animal
  • Drug Evaluation, Preclinical
  • Liver / blood supply*
  • Liver / drug effects
  • Liver / enzymology
  • Male
  • PPAR gamma / administration & dosage*
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / pathology
  • Reperfusion Injury / prevention & control*
  • Rosiglitazone
  • Thiazolidinediones / administration & dosage*

Substances

  • Cytokines
  • PPAR gamma
  • Thiazolidinediones
  • Rosiglitazone
  • Aspartate Aminotransferases