Factor VIIa inhibitors: target hopping in the serine protease family using X-ray structure determination

Bioorg Med Chem Lett. 2008 Aug 15;18(16):4533-7. doi: 10.1016/j.bmcl.2008.07.044. Epub 2008 Jul 16.

Abstract

Selective factor VIIa-tissue factor complex (FVIIa/TF) inhibition is regarded as a promising target for developing new anticoagulant drugs. Compound 1 was discovered from focused screening of serine protease-directed compounds from our internal collection. Using parallel synthesis supported by structure-based drug design, we identified peptidemimetic FVIIa/TF inhibitors (compounds 4-11) containing L-Gln or L-Met as the P2 moiety. However, these compounds lacked the selectivity of other serine proteases in the coagulation cascade, especially thrombin. Further optimization of these compounds was carried out with a focus on the P4 moiety. Among the optimized compounds, 12b-f showed improved selectivity.

MeSH terms

  • Blood Coagulation / drug effects
  • Chemistry, Pharmaceutical / methods*
  • Crystallography, X-Ray / methods
  • Drug Design
  • Factor VIIa / antagonists & inhibitors*
  • Humans
  • Kinetics
  • Models, Chemical
  • Molecular Conformation
  • Peptides / chemistry
  • Serine Endopeptidases / chemistry
  • Serine Endopeptidases / pharmacology*
  • Serine Proteinase Inhibitors / chemical synthesis*
  • Serine Proteinase Inhibitors / chemistry
  • Thromboembolism / drug therapy*
  • Thromboembolism / enzymology

Substances

  • Peptides
  • Serine Proteinase Inhibitors
  • Serine Endopeptidases
  • Factor VIIa