This paper concentrates on the segmentation of histological images of oral sub-mucous fibrosis (OSF) into its constituent layers. In this regard hybrid segmentation algorithm shows very interesting results. The segmentation results depict the superiority of hybrid segmentation algorithm (HSA) in comparison to region growing algorithm (RGA). In clinical sense, the presented method provides an automatic means for segmenting histological layers (reference class map provided by the expert). The method shows potential in mimicking clinical acumen to act as a support system to oncologist.