Abstract
Macrolide antibiotics modulate the quorum-sensing system of Pseudomonas aeruginosa. We tested the effect of macrolide antibiotics on the cell density-dependent expression of the MexAB-OprM efflux pump and found that 1.0 mug/ml (MIC/6.25) of azithromycin suppressed the expression of MexAB-OprM by about 70%, with the result that the cells became two- to fourfold more susceptible to antibiotics such as aztreonam, tetracycline, carbenicillin, chloramphenicol, and novobiocin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Azithromycin / pharmacology
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism*
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Down-Regulation / drug effects
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Humans
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Macrolides / pharmacology*
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Microbial Sensitivity Tests
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Pseudomonas Infections / drug therapy
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism*
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Pseudomonas aeruginosa / pathogenicity
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Quorum Sensing / drug effects
Substances
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Anti-Bacterial Agents
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Bacterial Outer Membrane Proteins
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Macrolides
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Membrane Transport Proteins
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MexA protein, Pseudomonas aeruginosa
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MexB protein, Pseudomonas aeruginosa
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OprM protein, Pseudomonas aeruginosa
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Azithromycin