Abstract
Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons and anterograde spread in the mammalian nervous system. Enhanced green fluorescent protein (GFP)-tagged Us9 mimicked the trafficking properties of the wild-type protein in nonneuronal cells. We constructed a pseudorabies virus strain that expressed Us9-GFP and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Axons / metabolism*
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Brain / cytology
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Brain / metabolism
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Cells, Cultured
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism*
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Herpesvirus 1, Suid / genetics
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Herpesvirus 1, Suid / metabolism*
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Intracellular Signaling Peptides and Proteins
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Lipoproteins / genetics
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Lipoproteins / metabolism*
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Male
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Membrane Microdomains / metabolism
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Neurons / cytology
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Neurons / metabolism
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Neurons / virology
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Protein Transport
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Pseudorabies / metabolism*
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Rats
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Rats, Sprague-Dawley
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism*
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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Intracellular Signaling Peptides and Proteins
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Lipoproteins
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Phosphoproteins
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Recombinant Fusion Proteins
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US9 protein, Suid herpesvirus 1
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Viral Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins