Abstract
Developing megakaryocytes are distinguished from progenitor cells by the appearance of platelet proteins such as platelet factor 4 (PF 4). The human erythroleukemic cell line HEL can also be induced to produce PF 4 by incubation in phorbol esters. HEL cells were used here as a model system in which to study the phenomenon of inducible PF 4 production at both the mRNA and protein levels. The cytokines interleukin 1 beta (IL-1 beta), interleukin 3 (IL-3), interleukin 6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoietin (EPO), and transforming growth factor-beta (TGF-beta) were also evaluated for their effects on PF 4 mRNA induction in HEL cells.
MeSH terms
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Antigens, Surface / analysis
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Blood Platelets / immunology
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Cell Line
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Cycloheximide / pharmacology
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Cytokines / pharmacology
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DNA / biosynthesis
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Gene Expression / drug effects
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Humans
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Leukemia, Erythroblastic, Acute / genetics*
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Platelet Factor 4 / genetics*
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Platelet Factor 4 / metabolism
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Platelet Membrane Glycoproteins / immunology
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Platelet Membrane Glycoproteins / metabolism
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics
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Tetradecanoylphorbol Acetate / pharmacology*
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Transforming Growth Factor beta / pharmacology*
Substances
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Antigens, Surface
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Cytokines
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Platelet Membrane Glycoproteins
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RNA, Messenger
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RNA, Neoplasm
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Transforming Growth Factor beta
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Platelet Factor 4
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DNA
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Cycloheximide
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Tetradecanoylphorbol Acetate