Preliminary X-ray crystallographic investigation of human parvovirus B19

Virology. 1991 Sep;184(1):170-4. doi: 10.1016/0042-6822(91)90833-w.

Abstract

Crystals that diffract X rays to at least 8 A resolution have been grown from human B19 parvovirus empty capsids. These particles consist of VP-2 derived from a baculovirus expression system. This is possibly the first time that a self-assembled empty viral capsid, grown in other than normal host cells, has been crystallized. Partial X-ray diffraction data have been collected using synchrotron radiation. The space group is P2(1)3 with a = 362 A. The particle position in the crystal cell is given, at least roughly, from packing considerations.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Genes, Viral
  • Humans
  • Insecta
  • Microscopy, Electron
  • Parvoviridae / genetics
  • Parvoviridae / ultrastructure*
  • Plasmids
  • RNA, Messenger / genetics
  • X-Ray Diffraction / methods

Substances

  • RNA, Messenger