The preservation capacity of water and fertility of several soilless cultivation substrates for Panax quinquefolium was compared in a large area. The data indicate that vermiculite mixed with sand (volume 1:1) is the most suitable soilless substrate for P. quinquefolium, for it is loose and features good preservation of fertility, water retaining, drainage, and air permeability. Timely analysis of nutrient contents in the substrates during cultivating period may serve as a guideline for correct application of fertilizer.