Frequent switching of Polycomb repressive marks and DNA hypermethylation in the PC3 prostate cancer cell line

Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12979-84. doi: 10.1073/pnas.0806437105. Epub 2008 Aug 27.

Abstract

Epigenetic reprogramming is commonly observed in cancer, and is hypothesized to involve multiple mechanisms, including DNA methylation and Polycomb repressive complexes (PRCs). Here we devise a new experimental and analytical strategy using customized high-density tiling arrays to investigate coordinated patterns of gene expression, DNA methylation, and Polycomb marks which differentiate prostate cancer cells from their normal counterparts. Three major changes in the epigenomic landscape distinguish the two cell types. Developmentally significant genes containing CpG islands which are silenced by PRCs in the normal cells acquire DNA methylation silencing and lose their PRC marks (epigenetic switching). Because these genes are normally silent this switch does not cause de novo repression but might significantly reduce epigenetic plasticity. Two other groups of genes are silenced by either de novo DNA methylation without PRC occupancy (5mC reprogramming) or by de novo PRC occupancy without DNA methylation (PRC reprogramming). Our data suggest that the two silencing mechanisms act in parallel to reprogram the cancer epigenome and that DNA hypermethylation may replace Polycomb-based repression near key regulatory genes, possibly reducing their regulatory plasticity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cellular Reprogramming
  • CpG Islands / genetics
  • DNA Methylation*
  • Epigenesis, Genetic
  • Gene Expression Regulation, Neoplastic
  • Genes, Neoplasm
  • Humans
  • Male
  • Models, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Polycomb Repressive Complex 1
  • Polycomb-Group Proteins
  • Prostatic Neoplasms / genetics*
  • Repressor Proteins / genetics*
  • Stem Cells / metabolism

Substances

  • CBX8 protein, human
  • Polycomb-Group Proteins
  • Repressor Proteins
  • Polycomb Repressive Complex 1

Associated data

  • GEO/GSE12334