PNA-peptide conjugates as intracellular gene control agents

Nucleic Acids Symp Ser (Oxf). 2008:(52):31-2. doi: 10.1093/nass/nrn016.

Abstract

Serum-stabilized PNA-internalization peptides (Pip) conjugated to PNA complementary to the 705 aberrant beta-globin splice site are able to correct splicing and increase luciferase production in Hela pLuc705 cells with sub microM EC(50) in the absence of a transfection agent. Inhibition of microRNA-122 in liver cells is achieved by treatment with complementary PNA containing just a few attached Lys residues, again without need of a transfection agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • HeLa Cells
  • Humans
  • Luciferases, Firefly / biosynthesis
  • Mice
  • MicroRNAs / antagonists & inhibitors*
  • MicroRNAs / metabolism
  • Muscular Dystrophy, Duchenne / genetics
  • Peptide Nucleic Acids / chemistry*
  • RNA Splicing*
  • beta-Globins / genetics

Substances

  • MicroRNAs
  • Peptide Nucleic Acids
  • beta-Globins
  • Luciferases, Firefly