Human herpesvirus 7 detection by quantitative real time polymerase chain reaction in primary cutaneous T-cell lymphomas and healthy subjects: lack of a pathogenic role

Br J Dermatol. 2008 Nov;159(5):1131-7. doi: 10.1111/j.1365-2133.2008.08811.x. Epub 2008 Sep 6.

Abstract

Background: Primary cutaneous T-cell lymphomas (CTCLs) are a heterogeneous group of lymphomas where the tumour population emerges within a multiple subclone pattern. Mycosis fungoides (MF) and Sézary syndrome (SS) are characterized by the expansion of clonal CD4+/CD45RO+ memory T cells. Lymphomatoid papulosis (LyP) is a chronic, lymphoproliferative disorder included in the CD30+ primary CTCL spectrum. Several studies have suggested a role of viral infection for super-antigenic activation of T lymphocytes; however, evidence of their association with CTCLs is still lacking. Human herpesvirus (HHV) 7 is a CD4+ T-lymphotropic herpesvirus; its restricted cellular tropism and the ability to induce cytokine production in infected cells could make it an important pathogenic cofactor in lymphoproliferative disorders.

Objectives: To investigate the presence of HHV7 DNA on CTCL and healthy skin donors (HD).

Methods: We used quantitative real time polymerase chain reaction to evaluate the potential pathogenic role of HHV7.

Results: Twenty-seven of 84 (32.1%) HD were positive for HHV7 DNA. Twenty-one of 148 (14.2%) patients with CTCLs were positive for HHV7 DNA: nine of 39 (23.1%) SS, six of 14 (42.9%) CD30+ CTCLs and six of 24 (25.0%) LyP, and HHV7 DNA was negative in all 71 patients with MF.

Conclusions: These results seem to exclude a pathogenic role of HHV7 in CTCLs, suggesting the possibility of skin as a latency site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Genes, T-Cell Receptor gamma / genetics
  • Herpesvirus 7, Human / genetics
  • Herpesvirus 7, Human / isolation & purification*
  • Humans
  • Lymphoma, T-Cell, Cutaneous / pathology
  • Lymphoma, T-Cell, Cutaneous / virology*
  • Male
  • Middle Aged
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Skin Neoplasms / pathology
  • Skin Neoplasms / virology*
  • T-Lymphocytes / immunology
  • Young Adult