Antibody response dynamics to the Plasmodium falciparum conserved vaccine candidate antigen, merozoite surface protein-1 C-terminal 19kD (MSP1-19kD), in Peruvians exposed to hypoendemic malaria transmission

Malar J. 2008 Sep 9:7:173. doi: 10.1186/1475-2875-7-173.

Abstract

Background: In high-transmission areas, developing immunity to symptomatic Plasmodium falciparum infections requires 2-10 years of uninterrupted exposure. Delayed malaria-immunity has been attributed to difficult-to-develop and then short-lived antibody responses.

Methods: In a study area with <0.5 P. falciparum infections/person/year, antibody responses to the MSP1-19kD antigen were evaluated and associations with P. falciparum infections in children and adults. In months surrounding and during the malaria seasons of 2003-2004, 1,772 participants received > or =6 active visits in one study-year. Community-wide surveys were conducted at the beginning and end of each malaria season, and weekly active visits were completed for randomly-selected individuals each month. There were 79 P. falciparum infections with serum samples collected during and approximately one month before and after infection. Anti-MSP1-19kD IgG levels were measured by ELISA.

Results: The infection prevalence during February-July was similar in children (0.02-0.12 infections/person/month) and adults (0.03-0.14 infections/person/month) and was negligible in the four-month dry season. In children and adults, the seroprevalence was maintained in the beginning (children = 28.9%, adults = 61.8%) versus ending malaria-season community survey (children = 26.7%, adults = 64.6%). Despite the four-month non-transmission season, the IgG levels in Plasmodium-negative adults were similar to P. falciparum-positive adults. Although children frequently responded upon infection, the transition from a negative/low level before infection to a high level during/after infection was slower in children. Adults and children IgG-positive before infection had reduced symptoms and parasite density.

Conclusion: Individuals in low transmission areas can rapidly develop and maintain alphaMSP1-19kD IgG responses for >4 months, unlike responses reported in high transmission study areas. A greater immune capacity might contribute to the frequent asymptomatic P. falciparum infections in this Peruvian population.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Antibodies, Protozoan / blood*
  • Child
  • Child, Preschool
  • Endemic Diseases / prevention & control
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin G / blood
  • Infant
  • Malaria Vaccines / immunology*
  • Malaria, Falciparum / epidemiology
  • Malaria, Falciparum / prevention & control*
  • Malaria, Falciparum / transmission
  • Merozoite Surface Protein 1 / immunology*
  • Middle Aged
  • Peru / epidemiology
  • Plasmodium falciparum / immunology*
  • Prevalence
  • Seroepidemiologic Studies

Substances

  • Antibodies, Protozoan
  • Immunoglobulin G
  • Malaria Vaccines
  • Merozoite Surface Protein 1