Abstract
Synthesis of four glycolipids with different number of lauroyl groups on glucose or cellobiose as scaffolds is described. Their immunological evaluations either admixed with or covalently linked to J8, a peptide antigen derived from the C-terminus of the antiphagocytic M-protein of group A streptococcus, are also investigated. Administration of mixtures of J8 and glycolipids to B10BR (H-2(k)) mice induced low-levels of J8-specific IgG antibodies. While glycolipopeptides, in which J8 was covalently linked to the synthetic glycolipids, demonstrated high-levels of antibody titers comparable with the co-administration of these glycolipopeptides with complete Freund's adjuvant, suggesting clearly the strong potency of the synthesized glycolipids as self-adjuvanting moieties.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Bacterial / immunology
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Antigens, Bacterial / metabolism
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Bacterial Outer Membrane Proteins / immunology
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Bacterial Outer Membrane Proteins / metabolism
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Bacterial Vaccines / administration & dosage*
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Bacterial Vaccines / immunology
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Bacterial Vaccines / pharmacology
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Carrier Proteins / immunology
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Carrier Proteins / metabolism
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Female
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Freund's Adjuvant / administration & dosage*
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Freund's Adjuvant / immunology
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Freund's Adjuvant / pharmacology
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Glycolipids / administration & dosage*
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Glycolipids / chemical synthesis
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Glycolipids / pharmacology
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Glycopeptides / administration & dosage*
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Glycopeptides / chemical synthesis
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Glycopeptides / pharmacology
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Immunoglobulin G / blood
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Immunoglobulin G / immunology
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Mice
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Molecular Sequence Data
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Phagocytosis
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Streptococcus / drug effects*
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Streptococcus / growth & development
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Streptococcus / immunology
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Vaccines
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Carrier Proteins
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Glycolipids
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Glycopeptides
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Immunoglobulin G
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streptococcal M protein
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Freund's Adjuvant