Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama; Kazuhisa Maeda; Masayuki Yamato; Ryuhei Hayashi; Takeshi Soma; Yasutaka Hayashida; Joseph Yang; Masayuki Shirakabe; Akifumi Matsuyama; Akihiko Kikuchi; Yoshiki Sawa; Teruo Okano; Yasuo Tano; Kohji Nishida

doi:10.1002/term.111

Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

J Tissue Eng Regen Med. 2008 Oct;2(7):445-9. doi: 10.1002/term.111.

Authors

Hiroaki Sugiyama¹, Kazuhisa Maeda, Masayuki Yamato, Ryuhei Hayashi, Takeshi Soma, Yasutaka Hayashida, Joseph Yang, Masayuki Shirakabe, Akifumi Matsuyama, Akihiko Kikuchi, Yoshiki Sawa, Teruo Okano, Yasuo Tano, Kohji Nishida

Affiliation

¹ Department of Ophthalmology, Osaka University Medical School, Osaka 565-0871, Japan.

PMID: 18792424
DOI: 10.1002/term.111

Abstract

We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / cytology*
Adipose Tissue / metabolism
Animals
Cell Culture Techniques / methods*
Cells, Cultured
Epithelial Cells / cytology
Epithelium / metabolism
Humans
Mesenchymal Stem Cells / cytology*
Mice
Microscopy, Phase-Contrast / methods
Models, Biological
NIH 3T3 Cells
Stem Cells
Tissue Engineering / methods