[Autophagy in fibrotic and postoperative remnant liver in rat]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008 Aug;30(4):421-5.
[Article in Chinese]

Abstract

Objective: To evaluate the characteristics of autophagy in fibrotic and postoperative remnant liver.

Methods: Male Wistar rats were randomly divided into three groups: control group; fibrosis group, which received the solution of CCl4 in oil twice a week for 5 weeks; and hepatectomy group, which underwent 70% hepatectomy. Liver tissues and plasma were harvested 18 hours after the surgery. The rats' general conditions and plasma liver function were observed. Histopathological characteristics and regeneration were observed with microscope and transmission electron microscope. Qualitative analysis of autophagosome was made base on the data from transmission electron microscope.

Results: Compared with the control group, plasma total protein and albumin level significantly decreased in the fibrosis group (P < 0.01). Proliferating cell nuclear antigen (PCNA) index was 85%-95% in the fibrosis group. Plasma alanine aminotransferase and aspartate aminotransferase levels significantly increased in the hepatectomy group compared with the control group (P < 0.01), while the autophagical index significantly decreased in both the fibrosis group and hepatectomy group compared with the control group (-95%, P < 0.01; -19%, P < 0.05, respectively). PCNA index was 20%-30% in the hepatectomy group.

Conclusions: Autophagy is weakened after fibrosis and hepatectomy, although it differs between these two processes. Proper regulation of autophagy may help facilitate the recovery of the residual liver function after hepatectomy.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Aspartate Aminotransferases / blood
  • Autophagy*
  • Disease Models, Animal
  • Hepatectomy
  • Humans
  • Liver / metabolism
  • Liver / pathology
  • Liver / physiopathology
  • Liver / surgery
  • Liver Cirrhosis / metabolism
  • Liver Cirrhosis / pathology
  • Liver Cirrhosis / physiopathology*
  • Liver Cirrhosis / surgery*
  • Male
  • Proliferating Cell Nuclear Antigen / metabolism
  • Random Allocation
  • Rats
  • Rats, Wistar

Substances

  • Proliferating Cell Nuclear Antigen
  • Aspartate Aminotransferases
  • Alanine Transaminase