Investigations into the relationship between the dopaminergic system and ascorbic acid in rat striatum

Neurosci Lett. 1991 Jun 10;127(1):34-8. doi: 10.1016/0304-3940(91)90888-z.

Abstract

Levels of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), ascorbic acid (AA) and dehydroascorbic acid (DHAA) were determined by HPLC in the striatum of male Wistar rats after single or repeated injections of apomorphine (1 mg/kg/day s.c.) and/or haloperidol (1 mg/kg/day i.p.), and 24 h after the last drug administration. Apomorphine significantly reduced the DOPAC/DA ratio and increased the DHAA/AA ratio; these ratio changes were significantly correlated (r = -0.9969, P less than 0.0005). Haloperidol greatly increased the DOPAC/DA ratio; the DHAA/AA ratio was also slightly increased, but there was no significant correlation. When apomorphine was associated with haloperidol, the resulting DOPAC/DA ratio was significantly lower than after haloperidol alone; the DHAA/AA ratio was also significantly reduced in contrast to the effect of apomorphine alone. It is concluded that a non-selective DA receptor activation mediates, in a correlated way, both the inhibition of DA turnover and the increase of AA oxidation in the rat striatum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apomorphine / pharmacology
  • Ascorbic Acid / metabolism*
  • Corpus Striatum / metabolism*
  • Dopamine / metabolism*
  • Dopamine Antagonists
  • Haloperidol / pharmacology
  • In Vitro Techniques
  • Male
  • Rats
  • Rats, Inbred Strains

Substances

  • Dopamine Antagonists
  • Haloperidol
  • Apomorphine
  • Ascorbic Acid
  • Dopamine