Morphological alterations of radicular dentine pretreated with different irrigating solutions and irradiated with 980-nm diode laser

Microsc Res Tech. 2009 Jan;72(1):22-7. doi: 10.1002/jemt.20638.

Abstract

Background: The topographical features of intraradicular dentine pretreated with sodium hypochlorite (NaOCl) or ethylenediamine tetraacetic acid (EDTA) followed by diode laser irradiation have not yet been determined.

Purpose: To evaluate the alterations of dentine irradiated with 980-nm diode laser at different parameters after the surface treatment with NaOCl and EDTA.

Study design: Roots of 60 canines were biomechanically prepared and irrigated with NaOCl or EDTA. Groups were divided according to the laser parameters: 1.5 W/CW; 1.5 W/100 Hz; 3.0 W/CW; 3.0 W/100 Hz and no irradiation (control). The roots were splited longitudinally and analyzed by scanning electron microscopy (SEM) in a quali-quatitative way. The scores were submitted to two-way Kruskal-Wallis and Dunn's tests.

Results: The statistical analysis demonstrated that the specimens treated only with NaOCl or EDTA (control groups) were statistically different (P < 0.05) from the laser-irradiated specimens, regardless of the parameter setting. The specimens treated with NaOCl showed a laser-modified surface with smear layer, fissures, and no visible tubules. Those treated with EDTA and irradiated by laser presented absence of smear layer, tubules partially exposed and melting areas.

Conclusions: The tested parameters of 980-nm diode laser promoted similar alterations on dentine morphology, dependent to the type of surface pretreatment.

MeSH terms

  • Dentin / drug effects
  • Dentin / radiation effects*
  • Dentin / ultrastructure*
  • Edetic Acid / pharmacology
  • Humans
  • Lasers, Semiconductor*
  • Microscopy, Electron, Scanning
  • Root Canal Irrigants / pharmacology
  • Root Canal Preparation / methods*
  • Sodium Hypochlorite / pharmacology
  • Tooth Root / drug effects
  • Tooth Root / radiation effects
  • Tooth Root / ultrastructure

Substances

  • Root Canal Irrigants
  • Edetic Acid
  • Sodium Hypochlorite