Abstract
Five continuous lymphoid suspension cell cultures were established by cultivation of lymphocytes from African green monkeys. The immunological typing revealed the mixed pattern of cultures consisting of B and T cell types. Two types of viruses: herpesvirus and retrovirus C-type were demonstrated by electron microscopy. Indirect immunofluorescence and molecular hybridization studies showed one of the viruses to be an EBV-related herpesvirus of green monkeys designated HVGM and the other to belong to H (S) TLV-1 group.
MeSH terms
-
Animals
-
Antibodies, Monoclonal
-
Cell Separation
-
Cells, Cultured / microbiology
-
Cells, Cultured / ultrastructure
-
Chlorocebus aethiops / microbiology*
-
DNA / genetics
-
DNA, Viral / genetics
-
Fluorescent Antibody Technique
-
Herpesviridae / genetics
-
Herpesviridae / isolation & purification*
-
Herpesviridae / ultrastructure
-
Human T-lymphotropic virus 1 / genetics
-
Human T-lymphotropic virus 1 / isolation & purification*
-
Human T-lymphotropic virus 1 / ultrastructure
-
Lymphocytes / microbiology*
-
Lymphocytes / ultrastructure
-
Microscopy, Electron
-
Nucleic Acid Hybridization
-
Virion / genetics
-
Virion / isolation & purification
-
Virion / ultrastructure
-
Virus Cultivation
Substances
-
Antibodies, Monoclonal
-
DNA, Viral
-
DNA