Abstract
Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma / metabolism
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Carcinoma / pathology
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Cell Line, Tumor
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Chromatography* / instrumentation
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Chromatography* / methods
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Chromatography, Affinity* / instrumentation
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Chromatography, Affinity* / methods
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Epidermal Growth Factor / metabolism
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Epidermal Growth Factor / pharmacology
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Humans
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Ion Exchange
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Models, Biological
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Phosphopeptides / analysis*
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Phosphopeptides / chemistry
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Phosphopeptides / isolation & purification*
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Static Electricity
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Substrate Specificity
Substances
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Phosphopeptides
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Epidermal Growth Factor