Abstract
A cefotaxime-resistant Klebsiella pneumoniae ML4313 was obtained from a patient from intensive care unit of Military hospital in Tunisia. This strain was resistant to beta-lactams, aminoglycosides, quinolones and phenicols, and tetracyclines. It was identified as producer of extended-spectrum beta-lactamases (ESBL) by double-disk synergy test between amoxicillin-clavulanate and cefotaxime, ceftriaxone, ceftazidime and aztreonam. The ESBL was identified as CTX-M-28 by sequencing of PCR products and by isoelectric focusing. The ESBL resistance was transferred by a 50kb plasmid. CTX-M-28 is closely related to CTX-M-15. This is the first description of this enzyme in Tunisia.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Cefotaxime / pharmacology*
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Cross Infection / epidemiology
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Cross Infection / microbiology
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DNA, Bacterial / genetics
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Drug Resistance, Multiple, Bacterial / genetics*
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Hospitals, Military
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Humans
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Intensive Care Units
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Klebsiella Infections / epidemiology
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Klebsiella Infections / microbiology*
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Klebsiella pneumoniae / classification
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Klebsiella pneumoniae / genetics
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Klebsiella pneumoniae / isolation & purification*
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Molecular Sequence Data
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Mutation, Missense
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R Factors / genetics
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tunisia / epidemiology
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beta-Lactam Resistance* / genetics
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beta-Lactamases / chemistry
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beta-Lactamases / genetics*
Substances
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Bacterial Proteins
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DNA, Bacterial
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CTX-M-28 beta-lactamase, Klebsiella pneumoniae
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beta-Lactamases
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Cefotaxime