Soluble P-selectin as a marker of in vivo platelet activation

Clin Chim Acta. 2009 Jan;399(1-2):88-91. doi: 10.1016/j.cca.2008.09.018. Epub 2008 Sep 21.

Abstract

Background: Platelets are the major source of circulating sP-selectin. Elevated levels of this protein have been found in many atherothrombotic disorders. Thus, we investigated whether sP-selectin dosage might reflect platelet function in patients with risk factors for or with established cardiovascular diseases and whether its levels can be modulated by aspirin therapy.

Methods: Plasma sP-selectin levels and light transmission platelet aggregometry (LTA) were analyzed in 152 outpatients. The effects of a 6-month aspirin therapeutic course on sP-selectin levels and LTA in 51 consecutive patients have been also investigated.

Results: Significant correlations were observed between sP-selectin and Mx% LTA in response to epinephrine (p=0.022) and arachidonic acid (p=0.006), or between sP-selectin and collagen lag-phase (p=0.016). Multiple regression analysis showed that the only predictors of sP-selectin levels were platelet number (p<0.001) and collagen-induced lag-phase (p<0.01). Aspirin-treated patients showed a significant reduction of sP-selectin levels by 13% (p=0.021) which significantly correlated with collagen-induced lag-phase (p=0.005).

Conclusions: sP-selectin dosage could be proposed as a reliable marker of platelet activation in patients with major atherosclerotic risk factors either in the absence of clinically overt disease, and might represent a valid tool to asses in vivo platelet behavior.

Publication types

  • Clinical Trial

MeSH terms

  • Aspirin / therapeutic use*
  • Biomarkers / blood
  • Cardiovascular Diseases / drug therapy*
  • Female
  • Humans
  • Male
  • Middle Aged
  • P-Selectin / blood*
  • Platelet Activation / drug effects*
  • Platelet Activation / physiology*
  • Platelet Aggregation Inhibitors / therapeutic use*
  • Risk Factors
  • Solubility
  • Time Factors

Substances

  • Biomarkers
  • P-Selectin
  • Platelet Aggregation Inhibitors
  • Aspirin